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cy3 labeled antisense probes  (Servicebio Inc)

 
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    Servicebio Inc cy3 labeled antisense probes
    Cy3 Labeled Antisense Probes, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 labeled antisense probes/product/Servicebio Inc
    Average 86 stars, based on 1 article reviews
    cy3 labeled antisense probes - by Bioz Stars, 2026-05
    86/100 stars

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    MEG3 interacts with FUBP3 to regulate VGF expression in GSCs. ( A ) Silver staining of proteins pulled down by MEG3 specific and <t>antisense</t> probes from T4121 GSCs lysates. ( B ) Immunoblotting validation of FUBP3 expression in proteins retrieved via MEG3 probes and antisense probes from T4121 GSCs lysates, with GAPDH serving as the control. ( C ) RT-qPCR analysis of MEG3 levels following RIP experiments on T4121 and T387 cell lysates using FUBP3 antibody, with IgG serving as the control. Data are presented as mean ± SD ( n = 3 per group). * p < 0.05. ( D ) RT-qPCR analysis of FUBP3 and VGF mRNA levels in T4121 and T387 GSCs with FUBP3 overexpression or knockdown. Data are presented as mean ± SD ( n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001. ( E ) Immunoblotting analysis of FUBP3 and VGF protein levels in T4121 and T387 GSCs with FUBP3 overexpression or knockdown
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    MEG3 interacts with FUBP3 to regulate VGF expression in GSCs. ( A ) Silver staining of proteins pulled down by MEG3 specific and antisense probes from T4121 GSCs lysates. ( B ) Immunoblotting validation of FUBP3 expression in proteins retrieved via MEG3 probes and antisense probes from T4121 GSCs lysates, with GAPDH serving as the control. ( C ) RT-qPCR analysis of MEG3 levels following RIP experiments on T4121 and T387 cell lysates using FUBP3 antibody, with IgG serving as the control. Data are presented as mean ± SD ( n = 3 per group). * p < 0.05. ( D ) RT-qPCR analysis of FUBP3 and VGF mRNA levels in T4121 and T387 GSCs with FUBP3 overexpression or knockdown. Data are presented as mean ± SD ( n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001. ( E ) Immunoblotting analysis of FUBP3 and VGF protein levels in T4121 and T387 GSCs with FUBP3 overexpression or knockdown

    Journal: Clinical and Experimental Medicine

    Article Title: MEG3 in glioma stem cells promotes glioblastoma angiogenesis through FUBP3-mediated VGF expression

    doi: 10.1007/s10238-026-02097-4

    Figure Lengend Snippet: MEG3 interacts with FUBP3 to regulate VGF expression in GSCs. ( A ) Silver staining of proteins pulled down by MEG3 specific and antisense probes from T4121 GSCs lysates. ( B ) Immunoblotting validation of FUBP3 expression in proteins retrieved via MEG3 probes and antisense probes from T4121 GSCs lysates, with GAPDH serving as the control. ( C ) RT-qPCR analysis of MEG3 levels following RIP experiments on T4121 and T387 cell lysates using FUBP3 antibody, with IgG serving as the control. Data are presented as mean ± SD ( n = 3 per group). * p < 0.05. ( D ) RT-qPCR analysis of FUBP3 and VGF mRNA levels in T4121 and T387 GSCs with FUBP3 overexpression or knockdown. Data are presented as mean ± SD ( n = 3 per group). * p < 0.05, ** p < 0.01, *** p < 0.001. ( E ) Immunoblotting analysis of FUBP3 and VGF protein levels in T4121 and T387 GSCs with FUBP3 overexpression or knockdown

    Article Snippet: Biotinylated antisense DNA probes (listed in Supplementary Table 2, synthesised by Sangon Biotech company) targeting MEG3 transcriptswere hybridized to crosslinked chromatin prepared from GSCs, followed by magnetic bead capture of associated nucleic acids.

    Techniques: Expressing, Silver Staining, Western Blot, Biomarker Discovery, Control, Quantitative RT-PCR, Over Expression, Knockdown